To build a molecular biology Lab needs to be equipped with an instrument introduction
refrigerator
The refrigerator is an essential instrument for storing reagents and samples in the laboratory. The most commonly used are: 4 ° C, -20 ° C refrigerator and -80 ° C ultra-low temperature refrigerator. Different reagents should be stored under the same conditions and should be stored at the correct temperature.
Specifically, items stored at different temperatures are as follows:
1) 4 ° C is suitable for storing certain solutions, reagents, medicines, etc.
2) -20 °C is suitable for certain reagents, drugs, enzymes, serum, formulated antibiotics and DNA, protein samples, etc.
3) -80 °C is suitable for the preservation of certain long-term cryopreserved samples, Escherichia coli strains, purified samples, special low-temperature treatment digestive juices, and competent conditions.
4) The 0-10 ° C chromatography freezer is suitable for electrophoresis, chromatography, dialysis and other experiments under low temperature conditions.
Liquid nitrogen tank
The preparation of competent cells in molecular biology experiments requires liquid nitrogen treatment. The preservation of the competent state can also be stored in liquid nitrogen.
Incubator
In molecular biology experiments, many reactions are carried out at specific temperatures, and a temperature-controlled device is required. For example, plate culture for bacteria, usually set at 37 ° C in the incubator inverted culture; other molecular biology experiments such as enzymatic digestion requires 25 ° C, 30 ° C, 37 ° C and other conditions.
Shaker
The shaker is a commonly used instrument in the laboratory, and generally has two types of normal temperature type and low temperature type. For molecular biology laboratories, if you can configure a low temperature shaker, you can adapt to different experimental needs. For example, it is used for shaking culture and protein induction expression of bioengineered strains such as Escherichia coli and yeast. The culture is usually 28 degrees and 37 degrees, and the expression is required to be 20-37 degrees. In the preparation process of the competent state, it is necessary to have 18 degree temperature control; used for protein gel staining when decolorizing, oscillating, used at room temperature; used for oscillation recovery in the conventional transformation of E. coli, usually 37 degrees. For controlled temperatures below room temperature, we need a low temperature shaker to control the temperature.
Water bath
The water bath is also a temperature control device for heat preservation and various types of experiments.
For example, a 25-100 ° C water bath shaker can be used for molecular hybridization tests, various biochemical enzyme reactions, etc. The low temperature water bath can be used for experiments such as the connection of plasmids and gene fragments in molecular biology and heat shock for 42 degrees of E. coli competent state.
Sterilizer
Most of the laboratory equipment used in molecular biology should be strictly sterilized. Including experimental items, reagents, culture media, and the like. Sterilizers are also available in different sizes, manual or fully automatic. Users need to choose according to their needs.
Oven
The oven is used for drying the experimental vessels after sterilization and washing. The oven has different temperature control ranges and the user can choose according to the experimental needs. For example, some plastic appliances can only be dried in an oven at 42-45 ° C; experimental equipment for RNA needs to be dried in a 250 ° C oven.
Pure water device
The pure water device includes a water distiller and a water purifier. The price of the distilled water is cheap, but it needs to be guarded in the process of water making; the price of the pure water machine is higher, but it is convenient to use, using tap water, distilled water, ion exchange water and reverse osmosis pure water as water supply, which is used for different levels of experiments. Pure water. For example, for PCR, PCR amino acid analysis, DNA sequencing, enzyme reaction, tissue and cell culture, etc., ultrapure water is required, and the general experimental water should be pure water.
Ice maker
The low temperature environment required for the experimental manipulation of most nucleic acids and proteins used in molecular laboratories to reduce the hydrolysis of nucleases or proteinases. Competent preparation also requires a long bath of ice. Snowflake ice machines are commonly used in laboratories. Ice machines are generally divided into different models according to the daily ice production.
Magnetic stirrer
Some reagents are more difficult to dissolve during reagent configuration, and a magnetic stirrer is required. A magnetic stirrer accelerates the dissolution of solid content. The magnetic stirrer generally has a heating function (related instruments: digital display heating type 7 inch square magnetic stirrer set).
Centrifuge
The centrifuge has freezing and room temperature. Mainly used for the preparation and collection of microbial cells, cell debris, large organelles and other sediments. Some samples require a low temperature environment because they are not stable at room temperature. For example, in a competing state preparation process, a low temperature environment must be ensured, so a centrifuge is required.
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