King No Stay - Identification
Identification
Medicine traits This product is spherical, about 2mm in diameter. The surface is black, a few red-brown, slightly shiny, with fine granular protrusions and a concave longitudinal groove on one side. Hard, white, endosperm curved, cotyledons 2. Gas slightly, slightly astringent, bitter.
Herbal microscopic identification of the product light grayish brown. Seed coat epidermis cells are reddish-brown or yellow-brown, with a polygonal or long polygonal surface, a diameter of 50-120 μm, a thickened vertical wall, a star-angled or deep-waved curve. The epidermis cells of the seed coat are yellow-brown and brown. The surface of the seed coat is square, rectangular or polygonal in shape, and the peristaltic wall is densely beaded and thickened. The surface shows a thickening texture in the form of a net. The endosperm cells are polygonal, square-like or oblong, and the cells are filled with starch grains and aleurone grains. Cotyledon cells contain fatty oil droplets.
Physical and chemical identification of medicinal herbs (1) Take 0.5g of this product powder, add 5ml of ethanol, warm the water bath for about 5 minutes, and filter. Take the filtrate 2ml, add a little magnesium powder, mix, drop a few drops of hydrochloric acid, that is, there are bubbles, while the solution gradient red. (Check flavonoids)
(2) take this product powder 1.5g, add methanol 20ml, heat reflux for 30 minutes, let cool, filter, the filtrate evaporated, the residue was dissolved in methanol 2ml, as the test solution. In addition, take the control line of 1.5g for the king of Vaccaria, and prepare the reference drug solution with the same method. According to the thin layer chromatography (Appendix VI B) test, 10 μl of each of the above two solutions was pipetted onto the same silica gel G plate, and the solution of chloroform-methanol-water (15:7:2) was used as the developing solution. , Unfold, remove, dry, and spray with modified potassium iodide test solution. In the chromatogram of the test sample, the same orange-red spot was observed at the position corresponding to the chromatogram of the reference drug.
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