8-OHdG/8-oxo-dG ELISA Kit - Highly Sensitive 8-Hydroxydeoxyguanosine Quantitative Detection Protocol
Oxidative stress is a state in which an oxidizing substance represented by an oxygen radical in the body or a cell generates and eliminates an imbalance or excessive intake of an exogenous oxidizing substance, and causes an oxidizing substance to accumulate in a cell to initiate an oxidation reaction. 8-hydroxy-2'-deoxyguanosine (8-OHdG), lipid peroxide, super 
Measurements of oxide dismutase, glutathione peroxidase, catalase, superoxide anion radicals, hydroxyl radicals, nitric oxide, etc., often reflect changes in oxidative damage and antioxidant capacity. As a stable and reliable biomarker, 8-OHdG can be found in urine and various diseased tissues or infected cells.
As a DNA oxidative damage product is a key marker widely used to study the mechanism of oxidative damage in diseases, 8-OHdG can be composed of polycyclic aromatic hydrocarbons (PAH) (such as hydrazine, diisobutyl phthalate, ethyl benzene, phenylacetic acid). Etc., other organic matter (mercaptoacetic acid, acrolein), heavy metals, and ultraviolet radiation are induced. The in vivo metabolic pathway that produces 8-OHdG is closely related to other important biochemical molecular metabolic processes. The appearance of 8-oxo-dG and its isoforms is associated with many degenerative disease states caused by reactive oxygen species. Therefore, 8-oxo-dG is a biomarker molecule for oxidative stress and is widely used as an indicator of ROS in various disease research. Common detection methods such as high performance liquid chromatography-electrochemical (HPLC-EC) and enzyme-linked immunosorbent assay (ELISA). Amy Jett recommends the 8-OHdG/8-oxo-dG ELISA kit from Trevigen, a cell damage assay specialist.
As an upgrade to the first generation kit, the second generation 8-oxo-dG ELISA Kit II provides a faster and more sensitive way to detect and quantify 8-oxo-dG in samples of DNA, saliva, urine and plasma. Competitive immunoassay method. 8-oxo-dG was pre-embedded in the 96-well plate of the kit. The kit also provides a 8-oxo-dG monoclonal antibody, an enzyme-labeled secondary antibody and the corresponding detection substrate and buffer, for the user to design a complete and powerful 8-oxo-dG detection program.
The 8-OHdG/8-oxo-dG ELISA assay kit uses colorimetric analysis and is quick and easy to operate. In addition, the standard 96 test specifications meet high-throughput testing needs. Highly sensitive detection antibodies and associated optimization reagents ensure a detection range of 13 nM to 200 nM (0·89 ng/ml to 56.7 ng/ml) and reach 2 nM (0·57 ng/ml) The sensitivity of the content of 8-OHdG.
Amy Jet provides you with a one-stop solution for cell pressure and DNA damage detection. These protocols include DNA double-strand break damage, comet assays, and SOD enzymes. In addition to 8-oxo-dG ELISA kits, there are 8-oxo-dG-specific antibodies for IF/ICC localization and other related products. If you are interested in the above products and solutions, please contact Amy Jie Technology Co., Ltd. inquires about experimental solutions related to cell damage and stress studies, or requests the latest product information.
Trevigen is a fast-growing US biotechnology company focused on oncology research products and services for apoptosis, DNA damage and repair, tumor cell function and behavior. As the general agent of Trevigen in China, Amy Jie and Trevigen provide Chinese researchers with the best and latest quality products and technical services in the fields of oxidative stress, cell damage and tumor cell behavior research.
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