The pharmacological effects of Awei
Pharmacological effects
First, anti-allergic effect
The volatile oil emulsion of Fructus schisandrae in Xinjiang can prevent allergic media release and mast cell degranulation, and can directly antagonize the contraction response of histamine and SRSA to airway smooth muscle.
1. Effect on passive cutaneous anaphylaxis (PCA) in mice: 26 mice were randomly divided equally
A Wei
A Wei
3 groups, 9 in each group. One group was slowly intravenously injected with 10 mg/kg cromolyn sodium 5 minutes before antigen challenge, and the other group was given intraperitoneal injection of 50 mg/kg ferulic volatile oil emulsion 30 minutes before antigen challenge. In physiological saline, the optical density and spot diameter of the three groups were compared. [1]
2. The effect of degranulation of rat periosteum mast cells: 10 rats were randomly divided into two groups and injected with 0.1 ml of rat antiserum diluted 1:20 into the subcutaneous tissue of normal cranial cranium. After injection for 24 hours, Antigen challenge was performed. The control group received intraperitoneal injection of physiological saline 30 minutes before the antigen challenge. The administration group was injected intraperitoneally with 50mg/kg ferulic volatile oil and water emulsion to determine the percentage of degranulation of the periosteal mast cells in the administration group and the control group. Wei can significantly inhibit the degranulation of rat calvarial mast cells. The control group was 72±7.5% and the Awei group was 31±2%. There was a significant difference between the two groups (SD, n=5, P<0.01). [1]
3. Effect on Rabbit Arthus Response: Rabbits were randomly divided into 2 groups, 3 in each group. The complete Freund's adjuvant was injected 4 weeks before the control group and the administration group, once a week. On the seventh day after the last injection, use l%
A Wei
A Wei
Ovalbumin 0.2ml/only, total 6 points. Observe the degree of swelling and edema diameter and degree of local skin within 1, 2, 4, 6, 8, 12, 24 hours after the point of antigenic attack. In the control group, the skin was congested, bleeding was fused, and it was positive (100%). However, in the Astragalus volatile oil-water emulsion group, there was almost no inflammation in the skin (a), which significantly inhibited the Arthus reaction in rabbits.
4. Effect on sensitized rabbit ileum: Five rabbits were sensitized with 1% ovalbumin and complete Freund's adjuvant. After 4 weeks, the carotid artery was cut off and lethal and the ileum was removed. Different doses of ferulic volatile oils and water emulsions were found to significantly inhibit the maximal contractile response of sensitized ileum induced by 1% ovalbumin in 1 ml.
5. Effect on lung perfusion of sensitized guinea pigs: 10 guinea pigs were randomly divided into 2 groups and sensitized according to Brocklehurst method. After 4 weeks, the carotid artery was excised and lethal. All lungs were taken by lung perfusion method. The lungs were washed and perfused with a constant temperature of 37° C. with oxygen-enriched K-share solution. Before the experiment, the administration group preliminarily perfused the ferulogen volatile oil emulsion for 1-3 minutes, measured the perfusion volume for 5 and 10 minutes, and then used 1 ml of 1% ovalbumin for antigenic attack to record the lung perfusion volume at the same time. It was found that the ferulic volatile oil emulsion The lungs that attacked the antigen did not change the irrigation flow.
6. Antagonism of sensitized guinea pig antigen: 12 guinea pigs were randomly divided into 2 groups. The guinea pigs sensitized with 10% ovalbumin aerosol were used to induce asthma. In order to inject the antigen with a constant mass of 400 mmHg, 50 mg/kg of ferulic volatile oil/water emulsion was intragastrically administered for 1 hour in a 4 L volume glass hood. The Wei group can significantly prolong the incubation period of asthma. The incubation period before treatment was 100 ± 14 seconds, which was significantly different from that before treatment.
7. Effect of anti-histamine and SRS-A on asthma: Eighteen guinea pigs were randomly divided into 3 groups of 6 mice. According to the previous method, 0.4% histamine and 2000 u/ml of SRS-A were used to induce asthma. The pre-drug incubation period was measured. Four hours later, they were administered with 1% ferulic water emulsion aerosol and 50 mg/kg intraperitoneal injection and oral administration. The results showed that the water extract of A. fruticosa was able to antagonize the asthmatic effects of histamine and SRS-A. This shows that the ferulic water emulsion has anti-asthmatic effects on asthma caused by histamine and SRS-A.
Second, the impact on the uterus
1. Inhibition of uterine smooth muscle contraction to 2%, the inhibitory effect is more pronounced, uterine contractions are completely suppressed. After rinsing for 10 minutes, after about 1.5 hours, the uterine muscle contraction recovered slightly, but the amplitude was small, the frequency was slow, and there was no significant improvement for several hours. With 10 units of oxytocin added as a control, the rhythm-induced excitability-enhanced wave pattern was shown. On this basis, 1 ‰Aiwei fluid was added again, and obvious inhibition phenomenon appeared again.
2. Anti-pregnancy effect: On the 6th day of pregnancy, 1 mg of ferulic preparation was injected intraperitoneally. Although there was a certain anti-pregnancy effect, the effect was not ideal. The use of ferulic preparation 10mg, 1 to 5 days after pregnancy continuous intraperitoneal injection, there is a significant anti-implantation effect. The experiment proved that although ferulic acid has a significant inhibitory effect on the spontaneous contraction of isolated mice and rabbits in vitro, it has antagonistic effects on uterine spasm contractions induced by pituitrin and ergometrine; The exoskeletons of the rabbit were excitable. After removal of rabbit ovaries, the isolated uterus shows that although ferulic and its spontaneous contraction also have inhibitory effects, it is weaker than the normal uterus of isolated pregnant rabbits, but it is significantly inhibited on isolated uteri injected with estradiol. The effect is similar to that of the normal uterus without isolated uterus, and the excitability of the isolated uterus injected with progesterone based on estradiol injection, and the non-pregnant rabbit injected with progesterone without normal ovary removal and normal pregnancy The experimental results of rabbits isolated from the uterus are consistent, and ferulic has a direct effect on uterine smooth muscle, and when the level of estrogen in the body is higher, the inhibitory effect of ferulic on the uterus is enhanced, and there are two opposites for rabbits who are pregnant and have no pregnancy. The effect may be related to the level of progesterone in the animal.
Third, anti-inflammatory effect
No matter whether subcutaneous injection or oral administration, the volatile oil emulsion of A. ferrum Xinjiang has a significant inhibitory effect on the rat foot swelling caused by carrageenin, and the increase of vascular permeability caused by the inflammatory mediators histamine and 5-HT. Similar to indomethacin and dexamethasone, it was shown that ferulic has a significant effect on the acute phase of inflammation caused by inflammation and may be related to its ability to antagonize inflammatory mediators. The product can inhibit the immune inflammatory response caused by Freund's adjuvant, suggesting that it can inhibit late-onset allergy. This is type IV allergy. The experiment also confirmed that ferulic can significantly inhibit delayed-type hypersensitivity (DTH) in mice sensitized by SRBC. This type of response is a type IV allergy caused by cellular immunity and is caused by T lymphocytes. Specific reactions mediated. In addition, ferulic can inhibit the conversion of peripheral blood lymphocytes and reduce the amount of 3H-TdR incorporation, further confirming that ferulic has a direct impact on lymphocyte immune function.
Fourth, the role of cells, plasma
1. Effect of cAMP and cGMP content in rat peritoneal macrophages (MNCs): 25 mg and 50 mg/kg of ferulic emulsion was intraperitoneally injected twice per day for 5 days. As a result, the ferulic emulsion significantly increased cAMP in the intraperitoneal MNC. Decreases cGMP and increases cAMP/cGMP ratio.
2. Effect of cAMP and cGMP content in mouse plasma and spleen tissue: Ferulic emulsion 25 and 50 mg/kg. The mice were fed intragastrically twice a day for 5 days to measure cAMP and cGMP in plasma and spleen tissues. RESULTS: Ferulic water emulsion significantly increased cAMP levels in plasma and spleen without significant effect on cGMP content, but its ratio increased.
3. Effect of cAMP and cGMP content in tracheal smooth muscle of guinea pigs: The guinea pigs were given 25 mg/kg and 50 mg/kg gavage twice a day for 5 days to determine the content of cAMP and cGMP in tracheal smooth muscle. The results showed that the water extract of A. fruticosa significantly increased the content of cAMP in tracheal smooth muscle and decreased the content of cGMP, which increased the cAMP/cGMP ratio.
Fifth, the role of the heart
The water extract and water-alcohol extract of A. simonii can reduce the heartbeat amplitude of the isolated frog heart and increase the heart rate.
Six, antibacterial and insecticidal effects
The ferulae decoction has inhibitory effect on human Mycobacterium tuberculosis in vitro (1:1600) and is used together with sulfur, betel nut and cinnamon. Formulating a decoction in advance to gavage mice can reduce the rate of adult development after the mice become infected with schistosomiasis.
[Content determination]
Take this product 5 ~ 10g, according to volatile oil assay (Appendix XD) determination.
The product contains no less than 10.0% volatile oil.
ã€Tropism of taste】Bitter, Sim, Warm. Return to the spleen and stomach.
ã€Indications】 Consumer product, scattered lice, insecticide. Used for meat stagnation, phlegm and blood stasis, abdominal mass, abdominal mass.
[Usage and dosage] 1 ~ 1.5g, into the pill and topical plaster.
[Note] pregnant women are disabled.
[Storage] closed, home cool and dry place.
ã€Excerpt】Chinese Pharmacopoeia
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