The principle, application and maintenance of Bole-BIO-RAD microplate reader
Bole-BIO-RAD microplate reader iMark repair this machine is the problem is that after power-on, press the power button, the system starts. The display has no characters, the mechanical structure motor does not have self-test movement action. The instrument can not start normally. Press the conventional test instrument power supply The circuit is normal, the motherboard can not work normally, the oscilloscope test address bus data bus waveform is not normal. Analyze the CPU peripheral circuit, find the damaged components. The motherboard signal is normal. Installed test, self-test normally passed. The simulation test runs normally, the instrument is repaired.
The BioRad microplate reader is a professional instrument for reading and analyzing the results of enzyme-linked immunosorbent assay (EIA) experiments. The enzyme-linked immunoreactivity is carried out by an enzyme-catalyzed chromogenic substrate coupled to an antigen or an antibody. The result of the reaction is shown in color, and the antibody or antigen to be tested in the specimen can be judged by the depth of the color developed, that is, the value of the absorbance. concentration. The microplate reader is actually a disguised special photoelectric colorimeter or spectrophotometer. The basic working principle is basically the same as the main structure and photoelectric colorimeter.
First, the working principle of the microplate reader;
The antigen or antibody is bound to the surface of a solid support and maintains its immunological activity. The antigen or antibody is linked to an enzyme to form an enzyme-labeled antigen or antibody, and the enzyme-labeled antigen or antibody retains both its immunological activity and the activity of the enzyme. The antigen-antibody complex formed on the solid phase carrier is separated from other substances by washing, and finally the amount of the enzyme bound to the solid phase carrier is proportional to the amount of the test substance in the sample. After the substrate of the enzyme reaction is added, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the test substance in the sample, so that qualitative or quantitative analysis can be performed according to the depth of the color reaction. Due to the high catalytic frequency of the enzyme, the reaction effect can be greatly amplified, so that the measurement method achieves high sensitivity.
Second, the application of the microplate reader;
The microplate reader is widely used in clinical laboratory, biological research, agricultural science, food and environmental science. Especially in recent years, due to the application of a large number of enzyme-linked immunoassay kits, the microplate reader is in the field of reproductive health. The application is more and more extensive, and at the same time promotes the improvement of reproductive health technology. At present, in the domestic blood station clinical laboratory, the microplate reader has become an important tool for ELISA determination. The use of microplate readers to study phenol oxidase in agriculture provides a theoretical basis for the development of new pest control agents targeting this enzyme. Fumonisin is a recently discovered structurally related toxin metabolite produced primarily by several fungi that has been listed by the World Health Organization as one of several mycotoxins that were first studied in recent years. Therefore, the rapid and efficient detection of fumonisins is particularly important. Optical measurement of microplates using a microplate reader at 450 nm or 630 nm filters can detect fumonisins and has important applications in food safety. The growth curve generally refers to the total number of colonies CFU/mI. Or logarithm of the total number of colonies, log CFU/ml. A curve over time that can be used to demonstrate microbial growth and metabolic patterns. At present, there are shortcomings such as poor immediacy, cumbersome operation and easy pollution when measuring the suspension by spectrophotometer. Since the microplate reader is a fast, accurate and high-flux instrument, we always choose to use a microplate reader. The OD value of the bacterial suspension is measured to improve work efficiency and save time.
The new full-wavelength reader Multiskan GO supports end-point method, dynamics and spectral scanning measurements, using xenon lamps as the light source, wavelength selection through advanced grating systems, wavelengths ranging from 200nm to 1000nm, full spectrum scanning in 1nm steps , including UV and NIR, can choose any wavelength freely. It has the dual function of microplate and cuvette measurement, and can be used as a full-wavelength microplate reader or as a full-wavelength spectrophotometer.
BioRad Service Commitment: Repair according to maintenance procedures and operating procedures to ensure the quality of maintenance.
1. Strictly control the quality of accessories, and eliminate the use of fake accessories to replace old accessories.
2. The service hotline is on duty 24 hours a day and responds within 24 hours. The maintenance workshop and the front desk reception are not closed. The maintenance user will follow the repair and establishment of the maintenance system and set up a repair team in time to arrive at the scene for repair.
3. Charges strictly enforce the Municipal Price Bureau and our company's charging standards, do not exaggerate the failure, to prevent arbitrary charges.
4. Remote customer fault diagnosis, technical fault solution, and brand mailing accessories are quickly processed. We will expedite the repair of your electrical appliances by our customers who have sent them to us for repairs.
5. The electrical appliances repaired by our center will be subject to warranty. If there is any problem in the warranty period due to the quality of the repair or the quality of the replacement parts, our center is responsible for the repair.
As long as you call the company's 24-hour maintenance hotline, the company will be the first time to serve you brand service goals: 0 defects, customers 100% satisfaction.
The company's purpose: experienced, quality assurance, safety first, customers, comfortable, peace of mind, rest assured.
The service center was established in 2005. Since its establishment, it has closely focused on the "professional" service standards, and promoted the development and growth of the company with the service spirit of "I use your heart, you are assured", and won the market and return with the service concept of "integrity and pragmatism". The society has enabled the company to develop steadily for many years in an extremely competitive market and achieved good economic and social benefits.
The maintenance personnel have the grades issued by the Ministry of Labor and have many years of experience in maintenance and accumulation. Not only has a wealth of maintenance experience, but also has a good professional quality and a high sense of responsibility. The maintenance service center always adheres to the tenet of “Going to the city, and seeking the letterâ€, and the spirit of “forge ahead, and constantly developâ€, the company will serve you in the first time!
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