Method for increasing the conception rate of donor ewes
First, the choice of donor ewes
1. Choose age between 1 and a half to 4 years old, having more than one birth, good sensation, no reproductive health, and a normal estrus cycle in the estrous season as a donor.
2, during the milking period must be weaned for 30 days, make up for nutrition, restore the public can choose to use.
3. If bred sheep are selected, they must be mature, healthy and free of reproductive disease, have more than two estrus history and normal cycle.
Second, the transplantation time arrangement
Sheep embryo transfer is usually arranged during the estrus season. From the end of September to the end of November in the general autumn, the estrus of the sheep grows vigorously from February to April in the early spring, and the endocrine activity is most active. Selecting this period is conducive to the progress of super-discharge work, and the estrus can be bred in time after wound healing. Artificial insemination should be used as far as possible during breeding, and the series of problems in the donor sheep, such as pollution-induced endometritis, can be detected without losing time. And vaginitis, abnormal estrus, and persistent corpus luteum. Take timely treatment measures to increase the conception rate.
Third, the surgical method and the number of uses
1. Using the tube-flushing method from 68 hours to 72 hours after mating. The use of donor sheep is preferably no more than twice, and once used, the birth of one child and then the use of the second time, can explain that the donor sheep did not leave reproductive disorders and other sequelae after flushing, guaranteeing the next time and receiving Body sheep support. Avoid economic losses.
2, the use of laparoscopic intrauterine flushing embryo method (7 days) is best but 3 times, if the uterus is not contaminated, knife wounds recover quickly, normal estrus cycle can be flushed embryos 1 times.
Fourth, pay attention to the process of red embryo
1. When the embryo is washed by surgery, the wool around the operation site must be scraped and sterilized strictly to prevent inflammation, leaving behind trouble and reducing the sheep's utilization rate.
2. After the estrus of the donor sheep is superseded, the estrus should be used for 10 to 12 hours at intervals of 1 to 3 times with fresh essence to prevent infection of the genital tract by too many times, affecting the next use and re-breeding the conception rate.
3, red embryo, the operation should be light and detailed, to prevent fallopian tube, fallopian tube umbrella and other parts of the bleeding, resulting in adhesions, blockage, so that the ewes lose fertility.
4. The antibacterial fluid used for washing wounds and blood during surgery was previously added with penicillin in physiological saline. It is better to change to normal saline and Lincomycin hydrochloride to prevent blisters on the surface of the uterus, resulting in decreased utilization and conception rate.
5. After flushing the embryo, dilute 800,000 international units of penicillin and 1 million international units of streptomycin into the uterus with 20 ml of normal saline to prevent contamination of the uterus during breeding and surgery, and to increase the amount of the next donor's sheep. Reuse rate and conception rate.
6, after the red embryo suture, use the index finger and middle finger to separate the peritoneum and the intestine to avoid suturing the intestine. When the peritoneum is sutured, the needle should be pulled out from the inside, the line should be tightened to prevent the peritoneal line from opening later, the sagging of the intestine and reoperation or donor sheep death, causing unnecessary losses.
V. Post-embryo post-breeding treatment
1. After the donor sheep has blasted the embryo, about 10 days from the time of estrus, 0.8 ml to 1 ml of cloprostenol is intramuscularly injected to dissolve the multiple corpus luteum on the ovary to facilitate estrus and prevent the formation of a persistent corpus luteum.
2, after the donor sheep embryo, because the body's exogenous hormones continue to a certain level, the first time after the heat, try not to breed, there will be part of the sheep estrus cycle disorders, ovulation is not normal, easy to bear. Some sheep have lower fertility rates even if they are mated. Therefore, it is more suitable for the second-phase breeding, and the rate of fertility of the intramuscular injection of LRH-A3 is higher at the time of mating.
3. When the donor sheep was estrus, it was found that the secretion was turbid, uterine inflammation, and vaginitis. The rinsing and anti-inflammatory treatments were performed after the treatment. Prevent pregnancy difficulties and delays missed breeding opportunities.
4. Sheep that were not estrus for a long time after hedging embryos can only be injected into the vagina with CIRD. After taking 13-day intramuscular injection of cloprostenol 0.8 ml, they can be taken out and estrus can be used for breeding. For the ewes in the late spring, as the weather is getting hotter and hotter, the long-lasting estrus should be based on the situation as far as possible without aphrodisiac breeding. Because of the anti-seasonal aphrodisiac, high temperatures can easily result in genital tract contamination of the ewe, and the conception rate is also low, so it can be taken off-season and seized at the right moments in the early autumn to greatly increase the utilization rate and conception rate.
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