Management of eggplant after grafting

The management within 1 week after grafting is of utmost importance, and care must be taken to ensure that the management of humidity and temperature is of particular importance. It must be done in strict accordance with the technical requirements.

1, humidity control

The first 3 days of the healing period of grafting generally needs to maintain the relative humidity of the air during the day to reach 95%. Generally, the following aspects should be noted:

First, a small shed must be individually capped on the artificial seedbed. After grafting, the seedlings must be closed with a small arch shed. First, it is necessary to create a favorable condition for moisturizing. The humidity must be lowered after grafting for 3 days. This is the key to success.

The second is to prevent the shed film from directly dripping onto the grafted seedlings. The shed is dome-shaped, allowing the water droplets on the shed film to flow along the edge of the rim. Do not shake the shed film easily to prevent it from falling off.

The third is to add moisture in a timely manner. Sprinkle water on the bed where the nutrition bowl is placed to ensure the humidity of the air. However, after 3 days, it is necessary to pay attention to the release of moisture, otherwise it is very easy to have wound rot.

2, temperature regulation

The suitable temperature should be maintained within the first 3 days after grafting, generally 25-30°C during the day and 17-20°C at night. Generally avoid direct sunlight from 10:00 am to 4:00 pm, using shading nets, non-woven fabrics and other shade.

Gradually reduce the temperature after 3 days, and gradually increase the lighting time. After 8 days, the small shed was removed and normal management was transferred. Through the seedlings that have completely survived the graft healing period, they can be managed by referring to the management method of common eggplant seedlings.

Grafting is like performing a "surgical major operation" on eggplant plants. The quality of grafted seedlings is directly related to the survival rate of grafted seedlings. Callus can be formed 24-30 hours after grafting. The moisture and nutrients absorbed by the 6-7 days rootstock flow into the scion, and the color of the scion begins to turn green. After about 10-15 days, a sound catheter tissue will form between the rootstock and the scion, and the grafted seedling will completely survive. This time you can enter regular management.

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Sanger Sequencing, which was used for DNA nucleotide sequencing, was created and rewarded Nobel Prize by Frederick Sanger in 1977.
How does the Sanger Sequencing work?
Firstly, we use the primer to have a short primer binding next to the region of interest on the DNA template. Then, the DNA polymerase starts building up from the primer by adding complementary nucleotides to the DNA strand.
To find the STOP that allows us to identify the base of the very end of the particular DNA fragment. Sanger sequencing principle is applied to removing an oxygen atom from the ribonucleotide. ddNTP (Dideoxynucleotide) is to put a wrench into a gear. The polymerase enzyme will no longer add normal nucleotides to this DNA chain. The extension would stop.
We can identify the Chain terminating nucleotide by a specific fluorescent dye. Superyears Genetic Analyzer can support fluorescent dye up to 6/8 colors to be exact.
Sanger sequencing results in the formation of extension products of various lengths terminated with dideoxynucleotides at the 3' end.
Capillary Electrophoresis separates the extension products.
An electrical current injects the molecules into a long capillary tube filled with a gel polymer.
During CE (Capillary Electrophoresis), negatively charged DNA fragments move forward to the Positive electrode.
The speed at which a DNA fragment migrates through the medium is inversely proportional to its molecular weight.
This process can separate the extension products by size at a resolution of one base.
A laser excites the dye-labeled DNA fragments as they pass through a tiny window at the end of the capillary.
The excited dye emits light at a characteristic wavelength that is detected by a light sensor.
The software can interpret the detected signal and translate it into a basecall.
A sequencing reaction is performed in the presence of all four terminated nucleotides, a pool of DNA fragments that are measured and separated base by base.
Data file showing the sequence of the DNA in a colorful electropherogram.

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