The medical effect of old mulberry
Medical function
[chemical composition] 1. The product coarse powder 3g, add ethanol 20ml, set on the water bath heating 10min, filtration, the filtrate for the following tests: 1 take the above filtrate 10ml, add a little magnesium powder and mix, add a few drops of hydrochloric acid, slight heat, test solution cherry red. (Check flavonoids) 2 Take the above filtrate, point on the filter paper, set the UV lamp (254nm) under observation, showed blue fluorescence, drop aluminum trichloride after the test solution, then bright yellow-green fluorescence. (Check flavonoids) 2. TLC: Take 2 g of coarse powder, add 10 ml of petroleum ether, reflux for 10 min, filter, and concentrate the filtrate to 1 ml.
3μl, with linoleic acid as control, were respectively spotted on the same silica gel thin layer plate, spread with benzene-ethyl acetate (80:20), sprayed 0.1% α-nitroso-β-indophenol sulfuric acid test solution, heated Color. The corresponding positions of the sample liquid chromatogram and the reference solution chromatogram show the same color spots. ã€Pharmacological effects】 1. Immune-enhanced mouse alpha-acetate naphthyl esterase (ANAE)-positive T-lymphocytes and spleen B-lymphocytes (hemolytic plaque-forming cells) gradually decreased with age. The daily dose of 12.5g (crude drug)/kg of mulberry decoction to LACA mice for 10 consecutive days can significantly increase T lymphocytes in mice of different age groups; however, the same dose of mulberry decoction can only increase the young age. The number of mouse B lymphocytes did not have a significant effect on aged mice older than 1 year. . The 3H-TdR incorporation lymphocyte transformation assay showed that the mulberry decoction has the effect of moderately inducing lymphocyte transformation. 2. The effects of Na+ and K-deca-ATPase activity on 3 to 24 months old BALb/c and LAC pure mice were fed with 12.5 g/kg mulberry decoction daily for 2 weeks. Except for 24 months old mice, compared with the same age control group, the activity of Na+, K+-ATPase in red blood cell membrane was significantly decreased. Na+, K+-ATPase is associated with the release of energy by the body and active transport of Na+ and K+. Mulberry can reduce the activity of the enzyme may be one of its mechanisms of yin.
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