Centella asiatica identification
Identification of raw herbs
Character identification
This product is often rolled into a group. Root cylindrical, length 2 ~ 4cm, diameter 1 ~ 1.5mm, the surface light yellow or grayish yellow. Stalks are slender, yellowish-brown, with fine longitudinal wrinkles. The leaves were mostly shrunken and broken, and the complete ones were flattened or kidney-shaped after flattening. They were 1 to 4 cm in diameter, grayish green, and coarse blunt teeth at the edges; petiole length was 3 to 6 cm and twisted. Umbrellas axillary, short. The double-hanging fruit is oblate, with prominent raised longitudinal ribs and fine netting, with short fruit stems. Gas slightly, tasteless.
Identification
(1) Transverse section of stem: Epidermal cells are round or nearly square. Below are 2 to 4 columns of thick-cornered cells. There are 6 to 7 externally flexible vascular bundles, and the phloem is a micro-lumbered fiber group with 2 to 3 rows of fine cells in the bundle. The xylem vessels are arranged radially. The pith is larger. Secretory tracts can be seen in the cortex and rays, and 5 to 7 secretory cells around the diameter of 23 to 34 μm.
Leaf surface view: The upper and lower epidermis are finely polygonal, with indeterminate or inequality stomata, less epidermis and more lower epidermis.
(2) Take 1g of this product powder, use ethanol 25ml, heat and reflux for 30 minutes, filter, the filtrate is evaporated to dryness, the residue is added with water 20ml to dissolve, and water-saturated n-butanol is shaken to extract 2 times, each time 15ml, merge with Ding The alcohol solution was washed with 15 ml of water saturated with n-butanol, the aqueous solution was discarded, and the n-butanol solution was evaporated to dryness. The residue was dissolved in 1 ml of methanol to prepare a test solution. In addition, the Centella asiatica reference substance was added and methanol was added to make a solution containing 1 mg per 1 ml as a reference solution. According to the thin layer chromatography (Appendix VI B) test, 5 μl of each of the above two solutions were pipetted on the same silica gel G thin-layer plate, using chloroform-methanol-water (7:3:0.5) as the developing agent. Expand, remove, dry, spray with 10% sulfuric acid in ethanol and heat at 105°C until the spots are clearly visible. In the chromatogram of the test sample, the spots of the same color appear on the positions corresponding to the chromatogram of the reference substance.
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