A new generation of flow cytometry - the advantages and characteristics of Stradedigm flow cytometry

2022-09-05 09:07:51
In 1973, the first FACS I flow cytometer was launched from BD. So far, the development of flow cytometry has been nearly 30 years old. In addition to traditional brands such as BD and Beckman Coulter, there are still new ones. The brand models are launched in the market such as Germany Partec, Millipore Guava, ABI and so on. In 2008, Stradedigm brought together the advantages of similar products in the current market. With the latest design and cutting-edge technology, the company launched the first flow cytometer product, the S1000 series, with up to 4 laser 12 parameters. Since then, the company has launched 2 laser 8 parameters SE500 in 2010, and launched 4 laser 18-parameter S1000 EX series in 2011 to meet the needs of different customers.
Founded in 2004, Stratedigm is located in Silicon Valley, USA, and has a strong technical team. Founder Shervin Javadi was originally the BD hardware manager and head of the flow system development department, and partnered with partner Rosa Chang to participate in the design and development of FACSCanto and FACSAria.
Strantiigm responds to customer needs, keeps pace with product trends, and strives to create high-end flow detection systems for users around the world. The Stratedigm flow cytometer can simultaneously stimulate 14-color fluorescence, detect 18 optical parameters, and achieve true multi-color analysis. It can detect polystyrene spheres with particle size as small as 0.16um, reaching the industrial level and easily separating. Fixed platelets; minimum loading volume of less than 20 ul, sample loss dead volume less than 8 ul, is the best choice for precious sample testing; Stradedigm's most compact model and performance design allows it to be placed in a biosafety cabinet It ranks among the best products in the market for the same volume and energy consumption, achieving the best price/performance ratio. At present, Stradigm customers have been distributed in various research institutions in the United States, such as: Harvard Medical School, Yale University, University of California Davis, University of Oklahoma, US Food and Drug Administration (FDA), US Environmental Protection Agency, Vaxiion Medical, Spherotech, etc. , deeply trusted by the majority of European and American customers.
“...The researchers are very pleased with the resolution, sensitivity and small size of the Stradedig S1000. Compared to the BD's 4-laser 8-color LSR or Fortessa, the S1000 is very competitive.” – Bari Holm Nazario, Stem Cell and Streaming Cytometry Research Center
"CellCapture software is by far the best streaming analysis software." - Ryan Duggan, Technical Director, University of Chicago Cancer Research Center
“Stratedigm's new flow cytometer platform was first offered to Vaxiion an opportunity to address the counting of unlabeled and fluorescently labeled bacterial particles, making a significant contribution to our product improvement.” – Michael J. Newman, Executive Vice President, Vaxiion Medical Co., Ltd.
Stratedigm product line
Platform
Configurations
Fluorophores
SE500
2 lasers;4 colors
FITC, PE, PerCP, APC
S1000
4 lasers;8 colors
FITC, PE, PerCP, PE-Cy7, APC, APC-Cy7, PE-Texas 615/30, Pacific Blue (445/60), Cascade Blue (445/60)
S1000EX
4 lasers;14 colors
FITC, PE, PerCP, PE-Cy7, APC, APC-Cy7, Alexa 700, Pacific Blue, Qdot605, PacO, PE-Texas 615/30, PE-Cy5.5 710/40; QD655 (676/29), QD705 (690/40), QD800 (740LP)
S1200EX
2 lasers;6 colors
FITC, PE, PerCP, PE-Cy7, APC, APC-Cy7
S1200EX
2 lasers;7 colors
FITC, PE, PerCP, PE-Cy7, APC, APC-Cy7, Alexa 700
S1300EX
3 lasers;8 colors
FITC, PE, PerCP, PE-Cy7, APC, APC-Cy7, Pacific Blue, AmCyan
S1300EX
3 lasers;10 colors
FITC, PE, PerCP, PE-Cy7, APC, APC-Cy7, Alexa 700, Pacific Blue, Qdot605, Pacific Orange
Why is Stratedigm so popular with European and American customers? What is special about it?
(1) The whole machine design has significant advantages
The optical bench is integrated with the Unibody to ensure the robustness and stability of the instrument, ensuring its normal operation even when moving or impacting. The unique HDF assembly eliminates the instability caused by vibration and temperature, reduces the influence of ambient temperature on the optical path system of the instrument, and ensures the accuracy and repeatability of the experiment (Note: the temperature change can affect the fluorescence intensity and CV by more than 25%); Reducing the use of some components, the Stratodigm is only about 100 pounds (including the flow plate) compared to the similar configuration of the product, which greatly reduces the weight of the flow cytometer itself and improves the convenience of movement.
The patented laser configuration, unique detection channel design and flow system design achieve the most compact models, making it the smallest of its kind, and can be placed in a biosafety cabinet to meet the special needs of users.
Due to the compact structure of the overall structure and function, the Stradedigm flow cytometer significantly reduces energy consumption, only about 100W (the power of the same type is often kilowatts), thus greatly reducing the heat dissipation, which is equivalent to a desktop computer. Minimize the effects of heat dissipation on the instrument's optical path system.
(2) Patented laser excitation system
Fully flexible and multi-laser configuration, standard up to 4 lasers, and special lasers can be customized according to requirements, and the lasers are independently controlled, which can simultaneously excite 14 colors of fluorescence and obtain 18 optical parameters. Multi-laser multi-point space independent excitation system completely eliminates inter-laser interference and improves data accuracy. The most stable solid-state laser and patented laser setting system are used to obtain a stable optical path, ensuring that the machine can be used as needed, reducing human variability and improving experimental repeatability.
Stratedigm flow cytometry laser and fluorescent dye reference table
laser
Attributes
PMT
parameter
488nm (standard)
40mW high power solid blue laser
1
FITC 530/30
2
PE 580/30
3
PE-Texas 615/30
4
PerCP/PE-Cy5 690/40
5
PE-Cy5.5 710/40
6
PE-Cy7 740LP
640nm (standard)
40mW high power solid red laser
1
APC 676/29
2
Alexa 700 690/40
3
APC-Cy7 740LP
532nm (standard)
561nm (custom)
50mW high power solid green laser
50mW high power solid yellow-green laser
1
PE 580/30
2
PE-Texas Red/MCherry 615/30
3
PerCP/PE-Cy5 690-40
4
PE-Cy5.5 710/40
5
PE-Cy7 740LP
405nm (standard)
372nm (custom)
50mW high power solid state purple laser
16mW high power solid state UV laser
1
Pacific Blue/DAPI (445/60)
2
Pacific Orange/AmCyan (540/40)
3
QD605 (615/30)
4
QD655(676/29)
5
QD705 (690/40)
6
QD800 (740LP)
(C) Unique fluorescence detection system
Firstly, the quartz cup flow detection cell technology is adopted, and the photo-adhesive coupling large numerical aperture objective lens collects the fluorescent signal to the maximum extent (the light collection efficiency is over 77%), thereby improving the excitation efficiency and the detection sensitivity. Secondly, it is freely pluggable. The optical filter expands the fluorescence detection parameters and eliminates the need to adjust the optical path after replacement, thus ensuring the convenience and flexibility of the customer, and the filter can be adjusted with the introduction of new dyes. The patented Smart Detect TM fluorescence detection design is the largest and most gratifying development of streaming technology in the past 20 years. It greatly reduces the size of the instrument, reduces the cost, and improves the fluorescence collection efficiency and detection sensitivity. High-end streaming analysis system. At present, the Stradedigm flow cytometer can simultaneously detect 14 colors of fluorescence and obtain 18 optical parameters for true multicolor analysis.
(four) superior detection performance
High throughput: 10,000 cells per second can be detected without missing any cells.
High sensitivity: < 100 MESF/FITC, < 30 MESF/PE; coefficient of variation CV < 2%.
High resolution: It can detect polystyrene spheres with a particle size of 0.16um, which is the best in industrial grade; it can easily distinguish 1um of cell particles; it can separate unfixed platelets.
(Fives)   Superior flow system
The patented ISO pressure system reduces fluid flow control costs and complexity while improving service reliability; patented dynamic sealing technology improves seal reliability and applicability while reducing operating costs; excellent flow system is greatly reduced The dead volume loss of the sample makes the Stradedigm flow cytometer with a minimum sample load of less than 20 ul, which is especially suitable for precious samples, such as nerve cell analysis experiments. With automatic flow cleaning mode, it can automatically clean the flow chamber and sample chamber, reduce manpower consumption, and ensure that the cross-contamination rate between samples is less than 0.1%. 4 liters of sheath liquid barrel, 4 liters of waste liquid barrel, 4 liters of cleaning liquid barrel, reducing the frequency of adding sheath liquid; optional automatic sheath liquid tank injection and waste liquid discharge device; integrated liquid flow tray does not increase the instrument footprint Compact structure. Switching machine and daily maintenance, one-button operation, automatic control.
Signal detection and transmission using continuous digital signals, is the most advanced all-digital circuit, detecting electronic dead time is 0, does not miss any signal; 24-bit fluorescence resolution makes data resolution, signal accuracy and resolution the highest, cells The grouping effect is better, the detection and statistics are more accurate, and it is especially suitable for analyzing cell populations with small number, small difference in fluorescence expression or continuous expression of fluorescence. Parallel test data results coefficient of variation CV < 2%, ahead of the market's 5% level of similar products, to ensure data accuracy and repeatability, to meet the flow cytometry single sample processing and save data information requirements, Analyze the sorting area with precise delineation.
Stratedigm's software system, which is selected from a variety of gated tools to store and analyze data, is efficient and simple. There are a variety of analysis methods to choose from, including histograms, contour maps, density maps, 2D point maps, etc., covering all groups. Flexible correction, real-time and quickly obtain the corrected data, and Stratemanager TM remote real-time processing equipment related issues. A new experience for file sharing, easy transfer and editing for Windows operating environments. The output of the analysis result is completely connected with excel and word, and the operation is convenient.
Automated loading of the A600HTAS system
(7) High-throughput automatic sample loading system
In order to achieve a large number of sample testing and reduce labor costs, Stradedigm also developed a high-throughput automated loading system A600HTAS. The system accommodates a range of titration plates (24-well plates, 96-well plates, 384-well plates, etc.) and 12 Eppendorf tubes. The synergistic work of 6 independent probes ensures the accuracy of sample absorption and distribution; through rapid suction, it provides unparalleled speed and precision, greatly reducing the microtiter plate processing time of the whole plate. 96-well plate samples only need 25 Minutes; sample absorption and mixing are carried out by a predetermined injection port suction mixing and/or mechanical mixing, and two or more reagents may be mixed in any one of the microtiter plates. According to the CellCapture software, the volume of the sample and the lotion was adjusted between 5 and 500 ul, and the samples were automatically cleaned with less than 1% cross-contamination. Temperature regulation between 8-40 °C using cellcapture software using solid state technology protects samples from long-distance transport sample deactivation. The incubation time is adjusted by CellCapture software to design experimental experiments for experimental temperature and/or incubation time. The preset module allows new and old users to master the optimal conditions of experimental design in the shortest time. The unique fluidic structure and unique 'over-absorption' characteristics greatly eliminate the loss of transport and dead volume, ensuring that all samples absorbed are transported to the flow cytometer to ensure complete analysis of precious samples. With barcode recognition, the microtiter plate can be tracked in real time, and the barcode scanner is integrated with the sample application system. With the CellCapture software, the high-throughput autoloading system is compatible with any Stradedigm S1000 or SE500 series flow cytometer.
Stratedigm flow cytometry complete solution system
All in all, Stratedigm responds to customer needs and meets product trends, using a series of patented technology designs to make the Stradedigm flow cytometer a high-end detection system for cell analysis. Regardless of its appearance or performance, regardless of its upgradeability or price, we can't deny that the Stradedigm flow cytometer has unparalleled superiority.

Neck Massage

Best Neck Massager,Shiatsu Neck Massager,Neck Relax Massager,Best Massage For Neck Pain

Shenzhen Jie Zhong Lian Investment Co., Ltd. , https://www.szmeizonscares.com