What are the methods of seabuckthorn seedlings? Seabuckthorn seedling technology
What are the methods of seabuckthorn seedlings? Seabuckthorn is a kind of deciduous shrub. It is planted in the northwest of China. The plant can withstand a minimum of -50 °C and can withstand up to 50 °C. It can be used not only for desert greening, but also for medicinal purposes. It has antitussive and phlegm and stomach. The effects of digestion, blood circulation and dispersal, and then Xiaobian will specifically talk about seabuckthorn seedling technology.
1 seeding method
1.1 Choosing good varieties
Choose fresh, pest-free seeds.
1.2 Sowing time
Seabuckthorn can be sown in spring, summer and autumn. The best sowing time is spring, and the soil temperature starts at 14-16 °C.
1.3 germination
Seabuckthorn should be soaked and germinated before sowing, disinfected with 0.6% potassium permanganate solution and then germinated. When the seed splits 2/3, it can be sown.
1.4 Sowing
The width of the broadcast is about 15 cm wide, and the row spacing is about 25 cm. The bottom of the ditch must be flat. The seeds are evenly spread into the broadcast frame, and the fine sand is covered with about 2 cm. It is properly rolled to make the soil and seeds fully contact. After sowing, cover the grass and spray water to keep the soil moist. After 5 to 7 days, most of the seedlings are unearthed. After 15 days, the seedlings can be released.
2 cutting method
Cutting time: March-September, spring is suitable for mid-to-late March or early April;
At the time of cutting: to confirm the true seabuckthorn, the growth and development of half of the lignified branches are intercepted 10cm, soaked in the agent for rooting, and the solution of barium butyric acid (IBA) is most ideal;
Cutting density: plant spacing is (10 ~ 15) × (5 ~ 10) cm, the second year of spring transplant, row spacing is (30 ~ 60) × (15 ~ 17) cm; with 1 ~ 2 years of asexual reproduction of afforestation, planting The row spacing is 4 meters x 2 meters.
3 Breeding and sampling of seabuckthorn
The induction effect of explants was different at different times. Generally, the survival rate of inoculated explants was higher around March, and the germination rate after inoculation was as high as 95% or 100%. After the inoculation in May, the pollution rate was relatively high, and there were many vitrified seedlings. After 7 to 9 months, the browning phenomenon was more serious after inoculation. It was highly susceptible to contamination after inoculation in September-December and January-February. The tissue was cut into tissue pieces of about 4 cm in length, and the tissue block was washed three times with PBS buffer and transferred into a culture flask, and the stopper was placed in a 36.5 ° C incubator for about 2 hours. Remove the culture bottle from the micro-cartridge, and hold the culture flask at 46 degrees. The bottom of the bottle is gently injected into the culture medium. After the cells are swallowed from the tissue block, the culture solution is added. The rooting rate can be 15 days. Up to 75% or more.
4 root cutting method
Select the plant with good quality and no pests and diseases as the mother plant. Before the germination, dig a groove of 30-40 cm wide and 40-50 cm deep in the outer canopy of the mother plant, and cut the roots of the seabuckthorn tree into the organic decomposed fertilizer with scissors. Each plant is about 75 kg, mixed with wet soil and filled into the ditch. After seabuckthorn root stimulation, a large number of plexiform root seedlings will be produced on the ground near the ditch in May-June. Therefore, it is necessary to strengthen the management of root saplings in time, and remove too many buds after germination to ensure the normal growth of seedlings. The second step is to blame the roots. After the autumn leaves and before the spring germination, the seedlings near the crown of the mother plant are graded, and each bundle of 50-100 plants is used with 50 mg/kg of barium butyric acid or ABT. Rooting powder, soaking the root system for 7-8 hours to promote hair roots. The nursery is applied to the base fertilizer, and each row is planted in 2 rows with a line spacing of 30 cm. When planting, the plexus root seedlings are cut and planted, and each seedling is better with 3 to 5 hair roots. After planting, step on the water to promote the growth of seedlings.
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