Nuclear Pearl Operation Technology
2018-05-24 18:00:49
With the cultivation of nuclear pearls, nucleation surgery is the key. It is directly related to the quality of pearls. I will now have a nuclear pearl surgery operation preoperative preparation, surgical operation, postoperative care 3 steps introduced.
1 Preoperative preparation
1.1 Preparation of surgical tools and drugs Surgical tools: mouthpieces, fixed mouth, scalpels, scissors, tweezers, small blades, anatomical plates, platelets, cotton balls, droppers, surgical frames, wound cutters, needles for delivery , channel needle, sender, rounder, etc. Pharmaceutical agents: 0.1% chlortetracycline, 5% phospholipids, 5 g/m3 furazolidone. When the stainless steel products are not used, they are protected by lubricating oil. Before use, the oil can be wiped off with CCl4 dip gauze, wiped with a damp cloth, and then soaked in hot soapy water for 10 minutes. Rinse with clean water and allow to dry. Glass products can be first immersed in 5%-10% sodium metasilicate for 20-30 minutes, then rinsed with water, or repeatedly scrubbed with 95% alcohol cotton balls. Bamboo products must be sterilized at high temperatures, and they should not be pests when they are not used. The rubber products are generally not used. When they are used, they can be boiled in dilute alkaline solution and rinsed with cold water after cooling.
1.2 Sputum Preparation for Small Sputum: It is required that the annual rings should be large, smooth and complete appearance, robust and disease-free. The pod should be about 10cm long, preferably 3-4 years old. Fertility beads: complete shell, no disease injury, the edge of a bright yellow soft edge, strong shell, water range, ax foot fat, complete mantle, female body spawning is not. The ratio of nymphs to small fleas is preferably 7:1-10:1.
1.3 The preparation of the bead core is best done with oyster shells. The thickness of the shell is required to be greater than 3mm, and the specific gravity is close to that of pearl, and the freshness is good. The shells are cut into square pieces at the time of manufacture, then roughed to remove the corners, then bleached and polished to produce smooth, smooth and pure white beads.
2 operation
2.1 preparation of small pieces of cells First, use a scalpel to cut off the front and rear adductor muscles of the small piece of palate to make the shell open. Remove the visceral mass, remove the ligaments and rinse several times with fresh water. Next, use a scalpel to insert between the inner and outer epidermis to evenly peel the inner and outer skins. Cut the epidermis, pull it back to the small piece (note that it cannot be pulled straight), remove the pigment band, cut the piece into square pieces, and finally drip 0.01 mol/l physiological saline. The entire process is as follows: sectioning → washing → splitting → taking a small piece → trimming → sectioning → drip care solution.
Requirements for small pieces of cells: ready-to-use, small pieces of cell viability (percentage of viable cells) must be more than 80%; small pieces of mucinous cells cannot be used; small pieces of cells are about 1/10 to 1/8 of the surface of the nucleus. Must be dyed with either methylene blue or eosin.
Small cell production note: The operating room should be quiet, sanitary; no formaldehyde, alcohol and other volatile substances; not allowed to open the fan to avoid direct sunlight, direct light; cell can not be wrong positive and negative, uniform thickness, non-adhesion tissue pieces, no damage Tools are strictly sterilized.
2.2 The insert will be washed pearls, ventilated upwards 15-20 min upward, add water to open the shells. Use a mouth sealer to fasten the two shells. Use an opener to widen the opening. Then wash the mantle for 3-5 times. Use a rake to pull the shank and axe foot to the side without inserting.
After the preparatory work was done, the official wounds were planted. The mandible is mainly mantled and the visceral corps can be inserted appropriately. The specific operation is as follows: wounds are made on the mantle with a wound needle, and then a channel needle is inserted into the channel from the wound; then the nucleus is sent to the wound with a nucleus feeder, and then the nucleus is pushed to the bottom with a channel needle; after the nucleus is fixed , The cell patch was attached to the bead core with a film feeding needle and the operation was completed. This is "first release." The "same release method" and "rear release method" are roughly the same, except that the order of the implantation of the cell chip and the bead core is different. After the operation, 0.1% chlortetracycline or 5% phosphoric acid solution can be used to prevent inflammation or wound infection. The entire operation process is as follows: row 蚌 → opening è¡ rinsing 鳃 鳃 鳃 创 → wounds → nucleus, inserts → disinfection of wounds.
Insertion Notes: Surgical movements should be gentle; Insertion nucleus sequence one after another, after the front end, the first edge after the center; must be the secretory surface of the small piece of cell that is attached to the nucleus of the outer skin; depth should be appropriate, triangular ridge 0.5-0.7 Cm, the folds are 0.7-1cm deep; the number should be moderate, triangular beaks, pleated crowns are 24-30 beads/only, no gingivae is 15-20 beads/only; wound nucleation should be "goods" Glyph arrangement; can not damage the outer epidermis; in principle, the pearl can not stay in the operating room overnight.
3 postoperative care
3.1 Nursing fistula after nucleus insertion should be put into the special resting pool as soon as possible. The rest pool requires good ventilation, good water quality, water depth of 1.5 to 2 m, micro-flowing water, and a flow rate of 0.1 m/s. During the break, measures such as “flattening, turning, hanging, and raising†can be taken to strengthen management. After 12-15 days, they are moved into the formal aquaculture waters for breeding.
3.2 The operation should not be performed at high temperatures, and the fleas themselves have repellent behaviors, that is, surgical worms have a phenomenon of "denucleation" in resting breeding. In this regard, the following measures can be taken to increase the rate of nucleation: a) Improve the level of operation and keep the size of the wound consistent with the size of the bead core; b) Inject an anesthetic at the base of the surgical axe, such as 0.5-1 ml of ethyl ether, once/ 2d, 3d consecutively; c) Stitching the wound; d) Adhering the wound with the wound adhesive; e) Bundling the double hull 10-15d; f) Strengthening the feeding and management, so that the surgical file can be recovered as soon as possible.
1 Preoperative preparation
1.1 Preparation of surgical tools and drugs Surgical tools: mouthpieces, fixed mouth, scalpels, scissors, tweezers, small blades, anatomical plates, platelets, cotton balls, droppers, surgical frames, wound cutters, needles for delivery , channel needle, sender, rounder, etc. Pharmaceutical agents: 0.1% chlortetracycline, 5% phospholipids, 5 g/m3 furazolidone. When the stainless steel products are not used, they are protected by lubricating oil. Before use, the oil can be wiped off with CCl4 dip gauze, wiped with a damp cloth, and then soaked in hot soapy water for 10 minutes. Rinse with clean water and allow to dry. Glass products can be first immersed in 5%-10% sodium metasilicate for 20-30 minutes, then rinsed with water, or repeatedly scrubbed with 95% alcohol cotton balls. Bamboo products must be sterilized at high temperatures, and they should not be pests when they are not used. The rubber products are generally not used. When they are used, they can be boiled in dilute alkaline solution and rinsed with cold water after cooling.
1.2 Sputum Preparation for Small Sputum: It is required that the annual rings should be large, smooth and complete appearance, robust and disease-free. The pod should be about 10cm long, preferably 3-4 years old. Fertility beads: complete shell, no disease injury, the edge of a bright yellow soft edge, strong shell, water range, ax foot fat, complete mantle, female body spawning is not. The ratio of nymphs to small fleas is preferably 7:1-10:1.
1.3 The preparation of the bead core is best done with oyster shells. The thickness of the shell is required to be greater than 3mm, and the specific gravity is close to that of pearl, and the freshness is good. The shells are cut into square pieces at the time of manufacture, then roughed to remove the corners, then bleached and polished to produce smooth, smooth and pure white beads.
2 operation
2.1 preparation of small pieces of cells First, use a scalpel to cut off the front and rear adductor muscles of the small piece of palate to make the shell open. Remove the visceral mass, remove the ligaments and rinse several times with fresh water. Next, use a scalpel to insert between the inner and outer epidermis to evenly peel the inner and outer skins. Cut the epidermis, pull it back to the small piece (note that it cannot be pulled straight), remove the pigment band, cut the piece into square pieces, and finally drip 0.01 mol/l physiological saline. The entire process is as follows: sectioning → washing → splitting → taking a small piece → trimming → sectioning → drip care solution.
Requirements for small pieces of cells: ready-to-use, small pieces of cell viability (percentage of viable cells) must be more than 80%; small pieces of mucinous cells cannot be used; small pieces of cells are about 1/10 to 1/8 of the surface of the nucleus. Must be dyed with either methylene blue or eosin.
Small cell production note: The operating room should be quiet, sanitary; no formaldehyde, alcohol and other volatile substances; not allowed to open the fan to avoid direct sunlight, direct light; cell can not be wrong positive and negative, uniform thickness, non-adhesion tissue pieces, no damage Tools are strictly sterilized.
2.2 The insert will be washed pearls, ventilated upwards 15-20 min upward, add water to open the shells. Use a mouth sealer to fasten the two shells. Use an opener to widen the opening. Then wash the mantle for 3-5 times. Use a rake to pull the shank and axe foot to the side without inserting.
After the preparatory work was done, the official wounds were planted. The mandible is mainly mantled and the visceral corps can be inserted appropriately. The specific operation is as follows: wounds are made on the mantle with a wound needle, and then a channel needle is inserted into the channel from the wound; then the nucleus is sent to the wound with a nucleus feeder, and then the nucleus is pushed to the bottom with a channel needle; after the nucleus is fixed , The cell patch was attached to the bead core with a film feeding needle and the operation was completed. This is "first release." The "same release method" and "rear release method" are roughly the same, except that the order of the implantation of the cell chip and the bead core is different. After the operation, 0.1% chlortetracycline or 5% phosphoric acid solution can be used to prevent inflammation or wound infection. The entire operation process is as follows: row 蚌 → opening è¡ rinsing 鳃 鳃 鳃 创 → wounds → nucleus, inserts → disinfection of wounds.
Insertion Notes: Surgical movements should be gentle; Insertion nucleus sequence one after another, after the front end, the first edge after the center; must be the secretory surface of the small piece of cell that is attached to the nucleus of the outer skin; depth should be appropriate, triangular ridge 0.5-0.7 Cm, the folds are 0.7-1cm deep; the number should be moderate, triangular beaks, pleated crowns are 24-30 beads/only, no gingivae is 15-20 beads/only; wound nucleation should be "goods" Glyph arrangement; can not damage the outer epidermis; in principle, the pearl can not stay in the operating room overnight.
3 postoperative care
3.1 Nursing fistula after nucleus insertion should be put into the special resting pool as soon as possible. The rest pool requires good ventilation, good water quality, water depth of 1.5 to 2 m, micro-flowing water, and a flow rate of 0.1 m/s. During the break, measures such as “flattening, turning, hanging, and raising†can be taken to strengthen management. After 12-15 days, they are moved into the formal aquaculture waters for breeding.
3.2 The operation should not be performed at high temperatures, and the fleas themselves have repellent behaviors, that is, surgical worms have a phenomenon of "denucleation" in resting breeding. In this regard, the following measures can be taken to increase the rate of nucleation: a) Improve the level of operation and keep the size of the wound consistent with the size of the bead core; b) Inject an anesthetic at the base of the surgical axe, such as 0.5-1 ml of ethyl ether, once/ 2d, 3d consecutively; c) Stitching the wound; d) Adhering the wound with the wound adhesive; e) Bundling the double hull 10-15d; f) Strengthening the feeding and management, so that the surgical file can be recovered as soon as possible.