Techniques for expanding Chlorella halodendron
2018-05-24 18:00:48
In order to meet the needs of crab breeding, experts introduced chlorella from the United States for expansion. The algal cells are single-celled with cell walls, chromoplasts and chloroplasts. Their protein content is more than 50%, fat is 10%, carbohydrates are about 25%, and vitamins A, B1, B2, B12, niacin and vitamin C, etc. are also included. It is the most suitable opening bait for marine economic animals such as crab, shrimp, and shellfish. The use of Chlorella as the basic bait in crab breeding not only improves the survival rate of nursery, but also plays a role in purifying water quality and reducing production costs. The price of the algae expansion technology is summarized as follows:
First, disinfection. Including the disinfection of culture utensils and places and disinfection of culture fluids. Glassware cultured as algal species, glass jars, and concrete pools for expansion and cultivation and the tools used in the cultivation process should be thoroughly disinfected. There are many methods for disinfection. Bleaching powder is generally used in production, and it is prepared to be soaked and washed with an aqueous solution containing 100-300 ppm of available chlorine, and then rinsed with water several times. As the preparation of water used for the first and second types of algae, boiled and sterilized as water, tap water or deep well water may be used as the culture liquid for expanding the culture. If the river is prepared with natural river water, it should be disinfected with 10ppm bleach. After sufficient aeration, prepare the culture solution.
Second, the preparation of the culture solution: 1 Preparation of artificial seawater The main components are: sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, potassium bromide, magnesium sulfate, boric acid and water. 2 Preparation of nutrient solution The main components are: sodium sulfate, EDTA-sodium, boric acid, manganese chloride, sodium dihydrogen phosphate, magnesium chloride, and vitamin B1, vitamin B12 and other trace elements and water. 3 Preparation of culture solution: In 1 ml of artificial seawater, 1 ml of prepared nutrient solution was added.
3. Inoculation: After the culture fluid is well sterilized, the inoculation culture can be performed. The alga species with strong vigor and strong growth are selected and inoculated at a ratio of 1:1 or 1:2 on a sunny day at 8-10 hours. Large inoculum size allows the algae species to dominate in the culture fluid and use biological antagonism to inhibit other algae, thus reducing the chance of contamination and shortening the algae cultivation cycle.
Fourth, training management:
1. Ventilation: According to the size and conditions of the culture fluid volume, different ventilation methods are used. Generally, shaking culture, air compressor aeration culture and artificial agitation culture are used. The purpose is to make the algae body fully exposed to carbon dioxide and light by aeration and stirring, and the algae body is evenly distributed in the culture liquid to accelerate its growth.
2. Light: When the light intensity is weak, the speed of photosynthesis is the same as that of respiration, and the cells do not proliferate; when the light is strong, the growth rate of the cells is accelerated, and it stops until the light intensity reaches light saturation. Therefore, in sunny days (especially in the summer, the training room may be shaded appropriately with bamboo curtains, curtains, sunshades, etc. (except during winter), and artificial light sources may be used for supplemental lighting when there is insufficient light in rainy days.
3. Temperature: It can grow well in the temperature range of 10°C～36°C. The optimum temperature is 25°C～26°C.
4. pH value: The optimum pH for reproduction is 6-8, and neutral acidity is the best. Since the algal cells multiply, causing the pH of the algae to rise or fall, the pH is often measured during the cultivation process and adjusted in time according to the changes.
5. Pest control: During the cultivation of Chlorella, it is necessary to observe whether the color of the algal fluid is normal, whether there is sedimentation and attachment to the wall, the liquid surface has a sterile film, and there are no signs of predator biological pollution. If there is any doubt, Signs can be confirmed with a microscope for further examination. The most common contamination is protozoa. If it occurs, it can be killed with 1ppm of bleach. In order to prevent contamination of pests and algae, the most effective method is to always create a suitable growth environment for the algae growth and to control the appropriate concentration of algae. When the concentration reaches 110 7/ml, it should be expanded in due course. Harvest and use.
First, disinfection. Including the disinfection of culture utensils and places and disinfection of culture fluids. Glassware cultured as algal species, glass jars, and concrete pools for expansion and cultivation and the tools used in the cultivation process should be thoroughly disinfected. There are many methods for disinfection. Bleaching powder is generally used in production, and it is prepared to be soaked and washed with an aqueous solution containing 100-300 ppm of available chlorine, and then rinsed with water several times. As the preparation of water used for the first and second types of algae, boiled and sterilized as water, tap water or deep well water may be used as the culture liquid for expanding the culture. If the river is prepared with natural river water, it should be disinfected with 10ppm bleach. After sufficient aeration, prepare the culture solution.
Second, the preparation of the culture solution: 1 Preparation of artificial seawater The main components are: sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, potassium bromide, magnesium sulfate, boric acid and water. 2 Preparation of nutrient solution The main components are: sodium sulfate, EDTA-sodium, boric acid, manganese chloride, sodium dihydrogen phosphate, magnesium chloride, and vitamin B1, vitamin B12 and other trace elements and water. 3 Preparation of culture solution: In 1 ml of artificial seawater, 1 ml of prepared nutrient solution was added.
3. Inoculation: After the culture fluid is well sterilized, the inoculation culture can be performed. The alga species with strong vigor and strong growth are selected and inoculated at a ratio of 1:1 or 1:2 on a sunny day at 8-10 hours. Large inoculum size allows the algae species to dominate in the culture fluid and use biological antagonism to inhibit other algae, thus reducing the chance of contamination and shortening the algae cultivation cycle.
Fourth, training management:
1. Ventilation: According to the size and conditions of the culture fluid volume, different ventilation methods are used. Generally, shaking culture, air compressor aeration culture and artificial agitation culture are used. The purpose is to make the algae body fully exposed to carbon dioxide and light by aeration and stirring, and the algae body is evenly distributed in the culture liquid to accelerate its growth.
2. Light: When the light intensity is weak, the speed of photosynthesis is the same as that of respiration, and the cells do not proliferate; when the light is strong, the growth rate of the cells is accelerated, and it stops until the light intensity reaches light saturation. Therefore, in sunny days (especially in the summer, the training room may be shaded appropriately with bamboo curtains, curtains, sunshades, etc. (except during winter), and artificial light sources may be used for supplemental lighting when there is insufficient light in rainy days.
3. Temperature: It can grow well in the temperature range of 10°C～36°C. The optimum temperature is 25°C～26°C.
4. pH value: The optimum pH for reproduction is 6-8, and neutral acidity is the best. Since the algal cells multiply, causing the pH of the algae to rise or fall, the pH is often measured during the cultivation process and adjusted in time according to the changes.
5. Pest control: During the cultivation of Chlorella, it is necessary to observe whether the color of the algal fluid is normal, whether there is sedimentation and attachment to the wall, the liquid surface has a sterile film, and there are no signs of predator biological pollution. If there is any doubt, Signs can be confirmed with a microscope for further examination. The most common contamination is protozoa. If it occurs, it can be killed with 1ppm of bleach. In order to prevent contamination of pests and algae, the most effective method is to always create a suitable growth environment for the algae growth and to control the appropriate concentration of algae. When the concentration reaches 110 7/ml, it should be expanded in due course. Harvest and use.